Hotspot

trnT(UGU)-trnL(UAA)

LSC · 45777-46297 · 521 bp

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Pi 0.00896
MarkerSeek score 60.80
Primer available yes
Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name
trnT(UGU)-trnL(UAA)
feature_type
igs
region
LSC
length_bp
521
variable_sites
7
pi
0.00896
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(UGU)-trnL(UAA)_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 571-583 bp 1.0 79.9
trnT(UGU)-trnL(UAA)_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 579-591 bp 1.0 79.3
trnT(UGU)-trnL(UAA)_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 603-615 bp 1.0 79.0
trnT(UGU)-trnL(UAA)_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 611-623 bp 1.0 78.4
trnT(UGU)-trnL(UAA)_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 585-597 bp 1.0 78.0