Hotspot

psaA-ycf3

LSC · 41401-42067 · 667 bp

Back to Phacellaria

Pi 0.0126

MarkerSeek score 67.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 667
variable_sites: 10
pi: 0.0126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AATTGCAGAGGCTTGGTTCA	50.0	45.0	57.9	58.0	760-874 bp	1.0	84.0
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGCAGAGGCTTGGTTCAA	50.0	45.0	57.9	58.0	759-873 bp	1.0	84.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGAAATTGCAGAGGCTTGGT	50.0	45.0	57.9	58.0	763-877 bp	1.0	84.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGCAGAGGCTTGGTTCAAT	50.0	45.0	57.9	58.0	758-872 bp	1.0	84.0
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	740-854 bp	1.0	83.7