Hotspot

trnD(GUC)-trnY(GUA)

LSC · 29839-30185 · 347 bp

Back to Phacellaria

Pi 0.0793

MarkerSeek score 82.90

Primer available yes

Label trnD(GUC)-trnY(GUA)

Feature Metadata

label_name: trnD(GUC)-trnY(GUA)
feature_type: igs
region: LSC
length_bp: 347
variable_sites: 32
pi: 0.0793
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD(GUC)-trnY(GUA)_p1	ATGATTGGATTCGTCGGGAC	GCAATACGTCTACGCTGGTT	50.0	50.0	57.8	58.4	420-473 bp	1.0	85.0
trnD(GUC)-trnY(GUA)_p2	ATGATTGGATTCGTCGGGAC	GTTGGCAATACGTCTACGCT	50.0	50.0	57.8	58.4	424-477 bp	1.0	84.9
trnD(GUC)-trnY(GUA)_p3	ATGATTGGATTCGTCGGGAC	TTGGCAATACGTCTACGCTG	50.0	50.0	57.8	58.4	423-476 bp	1.0	84.9
trnD(GUC)-trnY(GUA)_p4	ATGATTGGATTCGTCGGGAC	GGGGACGGACTGTAAATTCG	50.0	55.0	57.8	58.4	443-496 bp	1.0	84.8
trnD(GUC)-trnY(GUA)_p5	ATGATTGGATTCGTCGGGAC	GGGACGGACTGTAAATTCGT	50.0	50.0	57.8	57.6	442-495 bp	1.0	84.6