primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	ACTTTCAATAACTGGTAAACACT	ATAGAGACTGCGTGTGCTTG	23	20	30.4	50.0	54.1	58.0	0.0	0.0	6.863596	296	1357	1161-1161	1.000000	40.3
psbA_p2	psbA	2	ACACTACTACTAAATAAGACGGA	ATAGAGACTGCGTGTGCTTG	23	20	34.8	50.0	54.1	58.0	0.0	0.0	6.915573	296	1357	1143-1143	1.000000	40.3
psbA_p3	psbA	3	ACACTACTACTAAATAAGACGGAAG	ATAGAGACTGCGTGTGCTTG	25	20	36.0	50.0	55.9	58.0	0.0	0.0	7.059664	296	1357	1143-1143	1.000000	40.3
psbA_p4	psbA	4	AACACTACTACTAAATAAGACGGA	ATAGAGACTGCGTGTGCTTG	24	20	33.3	50.0	54.8	58.0	0.0	0.0	7.153478	296	1357	1144-1144	1.000000	40.3
psbA_p5	psbA	5	ACACTACTACTAAATAAGACGGAA	ATAGAGACTGCGTGTGCTTG	24	20	33.3	50.0	54.8	58.0	0.0	0.0	7.153478	296	1357	1143-1143	1.000000	40.3
trnK(UUU)_p1	trnK(UUU)	1	AATGGCTTGTGAATGACGGA	AGAGTTCTTGCTAGTTCATGT	20	21	45.0	38.1	57.8	54.6	0.0	0.0	4.610654	1596	4213	2706-2785	1.000000	46.6
trnK(UUU)_p2	trnK(UUU)	2	ATGGCTTGTGAATGACGGAA	AGAGTTCTTGCTAGTTCATGT	20	21	45.0	38.1	57.8	54.6	0.0	0.0	4.610654	1596	4213	2705-2784	1.000000	46.6
trnK(UUU)_p3	trnK(UUU)	3	AATGGCTTGTGAATGACGGA	AGTAGAGTTCTTGCTAGTTCATGT	20	24	45.0	37.5	57.8	57.4	0.0	0.0	4.796659	1596	4213	2709-2788	1.000000	45.1
trnK(UUU)_p4	trnK(UUU)	4	ATGGCTTGTGAATGACGGAA	AGTAGAGTTCTTGCTAGTTCATGT	20	24	45.0	37.5	57.8	57.4	0.0	0.0	4.796659	1596	4213	2708-2787	1.000000	45.1
trnK(UUU)_p5	trnK(UUU)	5	ATGGCTTGTGAATGACGGAA	AGAGTTCTTGCTAGTTCATGTT	20	22	45.0	36.4	57.8	55.4	0.0	0.0	4.804577	1596	4213	2705-2784	1.000000	45.0
matK-trnK(UUU)_p1	matK-trnK(UUU)	1	TGTTGCCGATATCTATCTCGT	AACGGTAGAGTACTCGGCTT	21	20	42.9	50.0	56.4	58.2	0.3	17.1	2.730453	3470	4176	775-778	0.666667	46.7
matK-trnK(UUU)_p2	matK-trnK(UUU)	2	TGTTGCCGATATCTATCTCGT	ACGGTAGAGTACTCGGCTTT	21	20	42.9	50.0	56.4	58.2	0.3	17.1	2.730453	3470	4176	774-777	0.666667	46.7
matK-trnK(UUU)_p3	matK-trnK(UUU)	3	TGTTGCCGATATCTATCTCGTT	AACGGTAGAGTACTCGGCTT	22	20	40.9	50.0	57.2	58.2	0.3	17.1	3.016982	3470	4176	775-778	0.666667	44.4
matK-trnK(UUU)_p4	matK-trnK(UUU)	4	TGTTGCCGATATCTATCTCGTT	ACGGTAGAGTACTCGGCTTT	22	20	40.9	50.0	57.2	58.2	0.3	17.1	3.016982	3470	4176	774-777	0.666667	44.4
matK-trnK(UUU)_p5	matK-trnK(UUU)	5	TGTTGCCGATATCTATCTCGT	GGGTTGCTAACTCAACGGTA	21	20	42.9	50.0	56.4	57.5	0.3	0.0	3.022791	3470	4176	788-791	0.666667	44.3
rps16-trnQ(UUG)_p1	rps16-trnQ(UUG)	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5904	7697	1832-1867	1.000000	84.3
rps16-trnQ(UUG)_p2	rps16-trnQ(UUG)	2	TGTCCTTCATTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.5	58.1	0.0	16.9	0.618121	5904	7697	1844-1879	1.000000	81.0
rps16-trnQ(UUG)_p3	rps16-trnQ(UUG)	3	TCCTTCATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.6	58.1	0.0	16.9	0.647653	5904	7697	1842-1877	1.000000	80.8
rps16-trnQ(UUG)_p4	rps16-trnQ(UUG)	4	TTCATTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.3	58.1	0.0	16.9	0.737605	5904	7697	1839-1874	1.000000	80.1
rps16-trnQ(UUG)_p5	rps16-trnQ(UUG)	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5904	7697	1836-1871	1.000000	79.5
psbM-trnD(GUC)_p1	psbM-trnD(GUC)	1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.8	58.5	0.0	5.4	2.706630	29039	29764	798-841	1.000000	65.4
psbM-trnD(GUC)_p2	psbM-trnD(GUC)	2	TGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	22	20	40.9	50.0	57.6	58.5	0.0	5.4	2.827699	29039	29764	805-848	1.000000	64.4
psbM-trnD(GUC)_p3	psbM-trnD(GUC)	3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	20	20	45.0	50.0	55.8	57.0	0.0	5.4	3.226784	29039	29764	799-842	1.000000	61.2
psbM-trnD(GUC)_p4	psbM-trnD(GUC)	4	TGGAACTAGAATGAACAGTGCA	GTTCAATTGGTCAGAGCACC	22	20	40.9	50.0	57.6	57.0	0.0	5.4	3.347853	29039	29764	806-849	1.000000	60.2
psbM-trnD(GUC)_p5	psbM-trnD(GUC)	5	GGAACTAGAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	22	20	45.5	50.0	57.1	58.5	0.0	5.4	3.413216	29039	29764	804-847	1.000000	59.7
trnD(GUC)-trnY(GUA)_p1	trnD(GUC)-trnY(GUA)	1	ATGATTGGATTCGTCGGGAC	GCAATACGTCTACGCTGGTT	20	20	50.0	50.0	57.8	58.4	0.0	1.7	0.603505	29839	30185	420-473	1.000000	85.0
trnD(GUC)-trnY(GUA)_p2	trnD(GUC)-trnY(GUA)	2	ATGATTGGATTCGTCGGGAC	GTTGGCAATACGTCTACGCT	20	20	50.0	50.0	57.8	58.4	0.0	1.9	0.603505	29839	30185	424-477	1.000000	84.9
trnD(GUC)-trnY(GUA)_p3	trnD(GUC)-trnY(GUA)	3	ATGATTGGATTCGTCGGGAC	TTGGCAATACGTCTACGCTG	20	20	50.0	50.0	57.8	58.4	0.0	1.7	0.604649	29839	30185	423-476	1.000000	84.9
trnD(GUC)-trnY(GUA)_p4	trnD(GUC)-trnY(GUA)	4	ATGATTGGATTCGTCGGGAC	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	57.8	58.4	0.0	0.0	0.591989	29839	30185	443-496	1.000000	84.8
trnD(GUC)-trnY(GUA)_p5	trnD(GUC)-trnY(GUA)	5	ATGATTGGATTCGTCGGGAC	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.615666	29839	30185	442-495	1.000000	84.6
trnT(GGU)-psbD_p1	trnT(GGU)-psbD	1	GGCGTAAGTCATCGGTTCAA	GACCGGACCAACCTACAAAA	20	20	50.0	50.0	58.3	57.7	0.0	9.5	0.542072	31256	32430	1256-1316	1.000000	80.6
trnT(GGU)-psbD_p2	trnT(GGU)-psbD	2	GGCGTAAGTCATCGGTTCAA	GAAAGAGCAAGAGACCGGAC	20	20	50.0	55.0	58.3	58.3	0.0	9.5	0.566813	31256	32430	1268-1328	1.000000	80.4
trnT(GGU)-psbD_p3	trnT(GGU)-psbD	3	GGCGTAAGTCATCGGTTCAA	CTGCGTAACCAGTCATCCAT	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.598731	31256	32430	1224-1284	1.000000	80.2
trnT(GGU)-psbD_p4	trnT(GGU)-psbD	4	GGCGTAAGTCATCGGTTCAA	AGACCGGACCAACCTACAAA	20	20	50.0	50.0	58.3	58.6	0.0	9.5	0.856056	31256	32430	1257-1317	1.000000	78.1
trnT(GGU)-psbD_p5	trnT(GGU)-psbD	5	GCCATGGTAAGGCGTAAGTC	GACCGGACCAACCTACAAAA	20	20	55.0	50.0	58.7	57.7	14.7	9.5	0.962539	31256	32430	1266-1326	1.000000	77.2
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	AATTGCAGAGGCTTGGTTCA	20	20	50.0	45.0	57.9	58.0	4.1	0.0	0.115695	41401	42067	760-874	1.000000	84.0
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	ATTGCAGAGGCTTGGTTCAA	20	20	50.0	45.0	57.9	58.0	4.1	0.0	0.115695	41401	42067	759-873	1.000000	84.0
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TGAAATTGCAGAGGCTTGGT	20	20	50.0	45.0	57.9	58.0	4.1	0.0	0.115695	41401	42067	763-877	1.000000	84.0
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGCAGAGGCTTGGTTCAAT	20	20	50.0	45.0	57.9	58.0	4.1	0.0	0.115695	41401	42067	758-872	1.000000	84.0
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	41401	42067	740-854	1.000000	83.7
trnT(UGU)-trnL(UAA)_p1	trnT(UGU)-trnL(UAA)	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	45777	46297	571-583	1.000000	79.9
trnT(UGU)-trnL(UAA)_p2	trnT(UGU)-trnL(UAA)	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	45777	46297	579-591	1.000000	79.3
trnT(UGU)-trnL(UAA)_p3	trnT(UGU)-trnL(UAA)	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	45777	46297	603-615	1.000000	79.0
trnT(UGU)-trnL(UAA)_p4	trnT(UGU)-trnL(UAA)	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	45777	46297	611-623	1.000000	78.4
trnT(UGU)-trnL(UAA)_p5	trnT(UGU)-trnL(UAA)	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	45777	46297	585-597	1.000000	78.0
trnF(GAA)-tRNA-Met(CAU)_p1	trnF(GAA)-tRNA-Met(CAU)	1	TGATGCTTCAGGAATGGTCG	CGCCGTATGAAAGCAGTACT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105850	47347	48371	1092-1156	1.000000	84.5
trnF(GAA)-tRNA-Met(CAU)_p2	trnF(GAA)-tRNA-Met(CAU)	2	CCTCGTGTCACCAGTTCAAA	CGCCGTATGAAAGCAGTACT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129325	47347	48371	1037-1101	1.000000	84.4
trnF(GAA)-tRNA-Met(CAU)_p3	trnF(GAA)-tRNA-Met(CAU)	3	AATGGTCGGGATAGCTCAGT	CGCCGTATGAAAGCAGTACT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.282715	47347	48371	1080-1144	1.000000	83.1
trnF(GAA)-tRNA-Met(CAU)_p4	trnF(GAA)-tRNA-Met(CAU)	4	ATGGTCGGGATAGCTCAGTT	CGCCGTATGAAAGCAGTACT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.282715	47347	48371	1079-1143	1.000000	83.1
trnF(GAA)-tRNA-Met(CAU)_p5	trnF(GAA)-tRNA-Met(CAU)	5	GAATGGTCGGGATAGCTCAG	CGCCGTATGAAAGCAGTACT	20	20	55.0	50.0	57.6	58.1	0.0	0.0	0.524674	47347	48371	1081-1145	1.000000	81.2
psbE-petL_p1	psbE-petL	1	CCAACCTGCAATGAATAGGGA	AGTCGTATCTTGCTCATACCA	21	21	47.6	42.9	58.0	56.3	0.0	0.0	3.745705	60540	61802	1456-1480	1.000000	51.2
psbE-petL_p2	psbE-petL	2	AGCAAAAGAACGTTCTCCTGT	AGTCGTATCTTGCTCATACCA	21	21	42.9	42.9	58.1	56.3	29.8	0.0	3.809430	60540	61802	1384-1408	1.000000	50.8
psbE-petL_p3	psbE-petL	3	ACGTTCTCCTGTACTTCCAGA	AGTCGTATCTTGCTCATACCA	21	21	47.6	42.9	58.1	56.3	0.0	0.0	3.855925	60540	61802	1375-1399	1.000000	50.4
psbE-petL_p4	psbE-petL	4	TCAGCAAAAGAACGTTCTCCT	AGTCGTATCTTGCTCATACCA	21	21	42.9	42.9	57.8	56.3	29.8	0.0	3.922662	60540	61802	1386-1410	1.000000	49.9
psbE-petL_p5	psbE-petL	5	CGTTCTCCTGTACTTCCAGAC	AGTCGTATCTTGCTCATACCA	21	21	52.4	42.9	57.8	56.3	0.0	0.0	3.947710	60540	61802	1374-1398	1.000000	49.7
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	GAGACGAGCCGTTTCTCATT	AGTATCGTCACCGCAGTAGA	20	20	50.0	50.0	58.0	58.0	9.8	0.0	0.036435	102658	102865	377-380	1.000000	81.0
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	GAGACGAGCCGTTTCTCATT	TATCGTCACCGCAGTAGAGT	20	20	50.0	50.0	58.0	58.0	9.8	0.0	0.036435	102658	102865	375-378	1.000000	81.0
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	102658	102865	322-325	1.000000	80.8
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	102658	102865	320-323	1.000000	80.8
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	GAGACGAGCCGTTTCTCATT	TCCCCTACAGTATCGTCACC	20	20	50.0	55.0	58.0	57.9	9.8	0.0	0.065301	102658	102865	385-388	1.000000	80.7
rpl32-trnL(UAG)_p1	rpl32-trnL(UAG)	1	GAGCTACTGAGGAACAACGG	ACCTATTTCACCATAGCGGC	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.258448	104467	105033	1123-1134	1.000000	81.9
rpl32-trnL(UAG)_p2	rpl32-trnL(UAG)	2	AGAATCGCGCAGAACAGAAT	ACCTATTTCACCATAGCGGC	20	20	45.0	50.0	58.0	57.7	8.7	0.0	0.271853	104467	105033	1324-1330	1.000000	81.4
rpl32-trnL(UAG)_p3	rpl32-trnL(UAG)	3	GAGCTACTGAGGAACAACGG	GTGCGGTTATTGCGCTTAAG	20	20	55.0	50.0	58.0	58.2	0.0	9.7	0.192134	104467	105033	1614-1615	0.666667	65.7
rpl32-trnL(UAG)_p4	rpl32-trnL(UAG)	4	AGAATCGCGCAGAACAGAAT	GTGCGGTTATTGCGCTTAAG	20	20	45.0	50.0	58.0	58.2	8.7	9.7	0.205539	104467	105033	1810-1811	0.666667	65.6
rpl32-trnL(UAG)_p5	rpl32-trnL(UAG)	5	TCGTATAGTGGCTCCGTTCT	GTGCGGTTATTGCGCTTAAG	20	20	50.0	50.0	58.0	58.2	0.0	9.7	0.238247	104467	105033	1510-1511	0.666667	65.4
ccsA-psaC_p1	ccsA-psaC	1	CGGGCTGCATAGTTATGGTT	ACGAACTGAGGACTGTGTTG	20	20	50.0	50.0	58.0	57.8	0.5	0.0	0.257750	106182	107494	1453-1459	1.000000	80.4
ccsA-psaC_p2	ccsA-psaC	2	CGGGCTGCATAGTTATGGTT	CACGAACTGAGGACTGTGTT	20	20	50.0	50.0	58.0	57.8	0.5	0.0	0.257750	106182	107494	1454-1460	1.000000	80.4
ccsA-psaC_p3	ccsA-psaC	3	CGGGCTGCATAGTTATGGTT	AAGAGATGTGAATCCGCCTG	20	20	50.0	50.0	58.0	57.7	0.5	0.0	0.355037	106182	107494	1428-1434	1.000000	79.7
ccsA-psaC_p4	ccsA-psaC	4	TCTATTCGAAATCGGGCTGC	ACGAACTGAGGACTGTGTTG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.359203	106182	107494	1465-1471	1.000000	79.6
ccsA-psaC_p5	ccsA-psaC	5	TCTATTCGAAATCGGGCTGC	CACGAACTGAGGACTGTGTT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.359203	106182	107494	1466-1472	1.000000	79.6
psaC-rps15_p1	psaC-rps15	1	CGTGGAGCAGAAGCAATTTG	TTGGGAAAACGTCAACGACT	20	20	50.0	45.0	58.0	58.0	0.0	9.9	0.053865	107741	108707	1157-1161	1.000000	86.4
psaC-rps15_p2	psaC-rps15	2	GCTTTACATCCCTCCCAAGG	TTGGGAAAACGTCAACGACT	20	20	55.0	45.0	57.9	58.0	0.0	9.9	0.085596	107741	108707	1133-1137	1.000000	86.3
psaC-rps15_p3	psaC-rps15	3	TCGTGGAGCAGAAGCAATTT	TTGGGAAAACGTCAACGACT	20	20	45.0	45.0	58.1	58.0	0.0	9.9	0.130784	107741	108707	1158-1162	1.000000	85.8
psaC-rps15_p4	psaC-rps15	4	TTCGTGGAGCAGAAGCAATT	TTGGGAAAACGTCAACGACT	20	20	45.0	45.0	58.1	58.0	0.0	9.9	0.130784	107741	108707	1159-1163	1.000000	85.8
psaC-rps15_p5	psaC-rps15	5	GACCCATACTGCGAGTTGTT	TTGGGAAAACGTCAACGACT	20	20	50.0	45.0	57.9	58.0	0.0	9.9	0.121766	107741	108707	1261-1265	1.000000	85.3
rrn5-rrn4.5_p1	rrn5-rrn4.5	1	AGTATCGTCACCGCAGTAGA	GAGACGAGCCGTTTCTCATT	20	20	50.0	50.0	58.0	58.0	0.0	9.8	0.036435	116138	116345	377-380	1.000000	81.0
rrn5-rrn4.5_p2	rrn5-rrn4.5	2	TATCGTCACCGCAGTAGAGT	GAGACGAGCCGTTTCTCATT	20	20	50.0	50.0	58.0	58.0	0.0	9.8	0.036435	116138	116345	375-378	1.000000	81.0
rrn5-rrn4.5_p3	rrn5-rrn4.5	3	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	116138	116345	322-325	1.000000	80.8
rrn5-rrn4.5_p4	rrn5-rrn4.5	4	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	116138	116345	320-323	1.000000	80.8
rrn5-rrn4.5_p5	rrn5-rrn4.5	5	TCCCCTACAGTATCGTCACC	GAGACGAGCCGTTTCTCATT	20	20	55.0	50.0	57.9	58.0	0.0	9.8	0.065301	116138	116345	385-388	1.000000	80.7