Hotspot

ycf1

SSC · 126442-132165 · 5724 bp

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Pi 0.00349

MarkerSeek score 42.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5724
variable_sites: 20
pi: 0.00349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAAACCAGAGCAGGTCTTT	GGGATCCGGACAAAATCGAT	45.0	50.0	57.0	57.7	259 bp	1.0	70.6
ycf1_p2	TTGAAACCAGAGCAGGTCTT	GGGATCCGGACAAAATCGAT	45.0	50.0	57.0	57.7	260 bp	1.0	70.6
ycf1_p3	TTTGAAACCAGAGCAGGTCT	GGGATCCGGACAAAATCGAT	45.0	50.0	57.0	57.7	261 bp	1.0	70.6
ycf1_p4	TGAAACCAGAGCAGGTCTTT	TCACCTTACAGAGACAGGCT	45.0	50.0	57.0	57.7	183 bp	1.0	70.6
ycf1_p5	TTGAAACCAGAGCAGGTCTT	TCACCTTACAGAGACAGGCT	45.0	50.0	57.0	57.7	184 bp	1.0	70.6