Hotspot

matK

LSC · 2080-3603 · 1524 bp

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Pi 0.00394

MarkerSeek score 61.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 6
pi: 0.00394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACTCAGTTGATTTACCCCTT	CCCTCAAAATCTTCCATCTTTGG	40.0	43.5	53.8	57.7	1659 bp	1.0	40.3
matK_p2	ACTCAGTTGATTTACCCCTT	TCCATCTTTGGTTCAAATCGA	40.0	38.1	53.8	55.5	1647 bp	1.0	40.3
matK_p3	ACTCAGTTGATTTACCCCTT	CTTCCATCTTTGGTTCAAATCGA	40.0	39.1	53.8	57.4	1649 bp	1.0	40.3
matK_p4	ACTCAGTTGATTTACCCCTT	TCTTCCATCTTTGGTTCAAATCG	40.0	39.1	53.8	57.4	1650 bp	1.0	40.3
matK_p5	ACTCAGTTGATTTACCCCTT	TCCATCTTTGGTTCAAATCGAA	40.0	36.4	53.8	56.3	1647 bp	1.0	40.3