Hotspot

ndhF-rpl32

SSC · 114790-115565 · 776 bp

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Pi 0.00644

MarkerSeek score 62.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 776
variable_sites: 5
pi: 0.00644
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCGAAAACTTTTTGTTGCCG	TGATGTCGAAGTGCGTTTTT	45.0	40.0	57.7	56.6	920 bp	1.0	66.4
ndhF-rpl32_p2	GCGAAAACTTTTTGTTGCCG	TTGATGTCGAAGTGCGTTTT	45.0	40.0	57.7	56.6	921 bp	1.0	66.4
ndhF-rpl32_p3	GCGAAAACTTTTTGTTGCCG	TTTGATGTCGAAGTGCGTTT	45.0	40.0	57.7	56.6	922 bp	1.0	66.4
ndhF-rpl32_p4	GCGAAAACTTTTTGTTGCCG	TTTTGATGTCGAAGTGCGTT	45.0	40.0	57.7	56.6	923 bp	1.0	66.4
ndhF-rpl32_p5	GCGAAAACTTTTTGTTGCCG	TTTTTGATGTCGAAGTGCGT	45.0	40.0	57.7	56.6	924 bp	1.0	66.4