Hotspot

rpl36-rps8

LSC · 82825-83251 · 427 bp

Back to Petunia

Pi 0.00937

MarkerSeek score 60.50

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 4
pi: 0.00937
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ACTATAATTCGTCCCCGCCT	GAGAGGCTCGACTAGAAGGA	50.0	55.0	58.3	57.7	554-555 bp	1.0	75.6
rpl36-rps8_p2	TTTATGTCTCGGGTTGGAGC	GAGAGGCTCGACTAGAAGGA	50.0	55.0	57.6	57.7	579-580 bp	1.0	74.7
rpl36-rps8_p3	GTCTCGGGTTGGAGCAAATT	GAGAGGCTCGACTAGAAGGA	50.0	55.0	58.5	57.7	574-575 bp	1.0	74.2
rpl36-rps8_p4	TATGTCTCGGGTTGGAGCAA	GAGAGGCTCGACTAGAAGGA	50.0	55.0	58.7	57.7	577-578 bp	1.0	72.0
rpl36-rps8_p5	TTATGTCTCGGGTTGGAGCA	GAGAGGCTCGACTAGAAGGA	50.0	55.0	58.7	57.7	578-579 bp	1.0	72.0