Hotspot

ndhC-trnV-UAC

LSC · 52604-53705 · 1102 bp

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Pi 0.00272
MarkerSeek score 60.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1102
variable_sites
3
pi
0.00272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCTCCGGCCCTTTGTTAATC CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.6 58.1 1256-1258 bp 1.0 76.0
ndhC-trnV-UAC_p2 TTTCTCCGGCCCTTTGTTAA CCGAGAAAGTCTACGGTTCG 45.0 55.0 57.3 58.1 1258-1260 bp 1.0 74.0
ndhC-trnV-UAC_p3 CCCTTTGTTAATCGGGGCTA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.3 58.1 1249-1251 bp 1.0 73.6
ndhC-trnV-UAC_p4 TTCTCCGGCCCTTTGTTAAT CCGAGAAAGTCTACGGTTCG 45.0 55.0 57.1 58.1 1257-1259 bp 1.0 72.2
ndhC-trnV-UAC_p5 CTCCGGCCCTTTGTTAATCG CCGAGAAAGTCTACGGTTCG 55.0 55.0 59.0 58.1 1255-1257 bp 1.0 71.4