Hotspot

trnG-GCC-trnfM-CAU

LSC · 38064-38296 · 233 bp

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Pi 0.0129

MarkerSeek score 59.90

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 3
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	335-336 bp	1.0	80.0
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	336-337 bp	1.0	80.0
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	299-300 bp	1.0	80.0
trnG-GCC-trnfM-CAU_p4	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	300-301 bp	1.0	80.0
trnG-GCC-trnfM-CAU_p5	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.9	58.0	376-377 bp	1.0	79.6