Hotspot

psbA

LSC · 288-1349 · 1062 bp

Back to Petrorhagia

Pi 0.00377

MarkerSeek score 46.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 4
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	TTGACACGGGCATATAAGGC	55.0	50.0	58.1	58.0	1419-1438 bp	1.0	81.0
psbA_p2	CAATCCACTGCCTTGATCCA	TTGACACGGGCATATAAGGC	50.0	50.0	57.9	58.0	1433-1452 bp	1.0	81.0
psbA_p3	AATCCACTGCCTTGATCCAC	TTGACACGGGCATATAAGGC	50.0	50.0	57.9	58.0	1432-1451 bp	1.0	80.9
psbA_p4	ACAATCCACTGCCTTGATCC	TTGACACGGGCATATAAGGC	50.0	50.0	57.9	58.0	1434-1453 bp	1.0	80.9
psbA_p5	GATCCACTTGGCTACATCCG	GAACTCGCGGGGATTCTATC	55.0	55.0	58.1	57.9	1863-1886 bp	1.0	80.4