Hotspot

ndhE-ndhG

SSC · 113518-113732 · 215 bp

Back to Petrorhagia

Pi 0.0419

MarkerSeek score 70.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 215
variable_sites: 9
pi: 0.0419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	CAAGACACATAAGAGCCCGA	GCAATTACTGTAGCGCGTCA	50.0	50.0	57.3	59.0	342-355 bp	1.0	68.7
ndhE-ndhG_p2	AGACACATAAGAGCCCGAAC	GCAATTACTGTAGCGCGTCA	50.0	50.0	57.3	59.0	340-353 bp	1.0	68.7
ndhE-ndhG_p3	GACACATAAGAGCCCGAACT	GCAATTACTGTAGCGCGTCA	50.0	50.0	57.3	59.0	339-352 bp	1.0	68.7
ndhE-ndhG_p4	TCAAGACACATAAGAGCCCG	GCAATTACTGTAGCGCGTCA	50.0	50.0	57.3	59.0	343-356 bp	1.0	68.6
ndhE-ndhG_p5	TTCGGCTCGTGATTAATCCA	GCAATTACTGTAGCGCGTCA	45.0	50.0	57.0	59.0	315-328 bp	1.0	66.4