Hotspot

rps4-trnT-UGU

LSC · 44240-44706 · 467 bp

Back to Petrorhagia

Pi 0.0516

MarkerSeek score 76.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 467
variable_sites: 24
pi: 0.0516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GCCCCTAAACGGCGTATTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.9	57.9	581-589 bp	1.0	75.3
rps4-trnT-UGU_p2	GCCCCTAAACGGCGTATTTT	GGTTCGATTCCGATAGCTGG	50.0	55.0	58.9	58.2	540-548 bp	1.0	74.9
rps4-trnT-UGU_p3	GCCCCTAAACGGCGTATTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.9	57.8	549-557 bp	1.0	74.6
rps4-trnT-UGU_p4	GCCCCTAAACGGCGTATTT	CTCAGAGGTTAGAGCATCGC	52.6	55.0	58.2	57.9	581-589 bp	1.0	72.8
rps4-trnT-UGU_p5	GCCCCTAAACGGCGTATTTT	CAGAGGTTAGAGCATCGCAT	50.0	50.0	58.9	57.5	579-587 bp	1.0	72.2