Hotspot

trnT-GGU-psbD

LSC · 29590-30628 · 1039 bp

Back to Petrorhagia

Pi 0.0273

MarkerSeek score 66.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1039
variable_sites: 28
pi: 0.0273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACGGTCTCTACGTAACCAGT	55.0	50.0	58.1	57.8	1179-1194 bp	1.0	80.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.1	57.8	1182-1197 bp	1.0	80.6
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.3	57.8	1156-1171 bp	1.0	79.1
trnT-GGU-psbD_p4	TGGTAGAGTAACGCCATGGT	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.4	57.8	1178-1193 bp	1.0	77.8
trnT-GGU-psbD_p5	CTCAGTGGTAGAGTAACGCC	ACGGTCTCTACGTAACCAGT	55.0	50.0	57.4	57.8	1183-1198 bp	1.0	76.8