Hotspot

rps16-trnQ-UUG

LSC · 5796-6336 · 541 bp

Back to Petrorhagia

Pi 0.053
MarkerSeek score 77.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
541
variable_sites
28
pi
0.053
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 635-644 bp 1.0 82.6
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GTAAGGCATCGGGTTTTGGT 45.0 50.0 58.1 58.5 636-645 bp 1.0 80.3
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GTGGTAAGGCATCGGGTTTT 45.0 50.0 58.1 58.5 639-648 bp 1.0 80.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TGGTAAGGCATCGGGTTTTG 45.0 50.0 58.1 58.5 638-647 bp 1.0 80.3
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.1 57.3 608-617 bp 1.0 78.9