Hotspot

trnH-GUG-psbA

LSC · 75-287 · 213 bp

Back to Petrorhagia

Pi 0.102

MarkerSeek score 76.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 20
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GGTGTAGCTGGTGTATTCGG	55.0	55.0	58.1	58.1	696-715 bp	1.0	82.9
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	ATATTCAGCTCCGGTTGCAG	55.0	50.0	58.1	58.0	859-878 bp	1.0	82.3
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	ATATTCAGCTCCGGTTGCAG	50.0	50.0	57.9	58.0	873-892 bp	1.0	82.2
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	ATATTCAGCTCCGGTTGCAG	50.0	50.0	57.9	58.0	872-891 bp	1.0	82.2
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	ATATTCAGCTCCGGTTGCAG	50.0	50.0	57.9	58.0	874-893 bp	1.0	82.2