Hotspot

ycf1

SSC · 123293-127975 · 4683 bp

Back to Petrocosmea

Pi 0.0169

MarkerSeek score 53.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4683
variable_sites: 242
pi: 0.0169
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCGCGATCCAGGTTTCAAT	TGACGGATCTGACTGATGGA	45.0	50.0	57.7	57.9	1132-1153 bp	0.75	71.7
ycf1_p2	GATCGCGATCCAGGTTTCAA	TGACGGATCTGACTGATGGA	50.0	50.0	58.3	57.9	1133-1154 bp	0.75	71.6
ycf1_p3	AAGGGTGTCTTTGTGTGTGT	TGACGGATCTGACTGATGGA	45.0	50.0	57.5	57.9	980-1001 bp	0.75	70.9
ycf1_p4	AGGGTGTCTTTGTGTGTGTT	TGACGGATCTGACTGATGGA	45.0	50.0	57.5	57.9	979-1000 bp	0.75	70.9
ycf1_p5	GGCAGTGGCGAACTCTATTT	TGACGGATCTGACTGATGGA	50.0	50.0	58.3	57.9	717-723 bp	0.625	66.2