Hotspot

rpl32-trnL-UAG

SSC · 112918-113785 · 868 bp

Back to Petrocosmea

Pi 0.0223

MarkerSeek score 67.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 868
variable_sites: 61
pi: 0.0223
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGACGAAGGTCTTTTGTTGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1550-1991 bp	1.0	87.0
rpl32-trnL-UAG_p2	CGACGAAGGTCTTTTGTTGC	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	2021-2462 bp	1.0	86.4
rpl32-trnL-UAG_p3	CGACGAAGGTCTTTTGTTGC	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.0	58.1	2022-2463 bp	1.0	86.4
rpl32-trnL-UAG_p4	GAAGGGGTATTGTGCATCGT	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1480-1499 bp	1.0	85.3
rpl32-trnL-UAG_p5	GAAGGGGTATTGTGCATCGT	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.0	58.1	1481-1500 bp	1.0	85.2