Hotspot

ndhC-trnV-UAC

LSC · 51204-52202 · 999 bp

Back to Petrocosmea

Pi 0.0192

MarkerSeek score 67.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 54
pi: 0.0192
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCAAAATGGGAATAAGACTTG	AAAGATGCACGTTGGGTCTT	39.1	45.0	57.6	58.0	681-1240 bp	1.0	60.7
ndhC-trnV-UAC_p2	TGCCAAAATGGGAATAAGACTTG	AAGATGCACGTTGGGTCTTT	39.1	45.0	57.6	58.0	680-1239 bp	1.0	60.7
ndhC-trnV-UAC_p3	TGCCAAAATGGGAATAAGACTTG	AAAAGATGCACGTTGGGTCT	39.1	45.0	57.6	58.0	682-1241 bp	1.0	60.6
ndhC-trnV-UAC_p4	TGCCAAAATGGGAATAAGACTTG	GCACGTTGGGTCTTTGAAAC	39.1	50.0	57.6	58.2	675-1234 bp	1.0	59.5
ndhC-trnV-UAC_p5	TGCCAAAATGGGAATAAGACT	AAGATGCACGTTGGGTCTTT	38.1	45.0	55.4	58.0	680-1239 bp	1.0	59.4