Hotspot

ycf3-trnS-GGA

LSC · 44952-45827 · 876 bp

Back to Petrocosmea

Pi 0.0154

MarkerSeek score 65.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 42
pi: 0.0154
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	47.6	50.0	57.0	58.1	936-982 bp	1.0	70.5
ycf3-trnS-GGA_p2	CCATTTATTCGCGATCTAGGC	ACGGAAAGAGAGGGATTCGA	47.6	50.0	57.0	58.2	957-1003 bp	1.0	69.9
ycf3-trnS-GGA_p3	CCATTTATTCGCGATCTAGGC	TTCCAATGCTACGCCTTGAA	47.6	45.0	57.0	57.8	907-953 bp	1.0	69.9
ycf3-trnS-GGA_p4	CCATTTATTCGCGATCTAGGC	ACCCTCGGTAAACAAAAGCC	47.6	50.0	57.0	58.4	937-983 bp	1.0	68.1
ycf3-trnS-GGA_p5	TCCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	45.5	50.0	58.4	58.1	937-983 bp	1.0	67.2