Hotspot

psbI-trnS-GCU

LSC · 8382-8498 · 117 bp

Back to Petrocosmea

Pi 0.0296
MarkerSeek score 63.90
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
117
variable_sites
9
pi
0.0296
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAAGA TGGACTAAAGCGTCGGATTG 50.0 50.0 57.1 58.0 213-214 bp 1.0 76.2
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAAGA GTGGACTAAAGCGTCGGATT 50.0 50.0 57.1 58.0 214-215 bp 1.0 76.2
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAAGA ATTGGGAGAGATGGCTGAGT 50.0 50.0 57.1 58.1 232-233 bp 1.0 75.1
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAAGAA TGGACTAAAGCGTCGGATTG 47.6 50.0 57.8 58.0 213-214 bp 1.0 73.9
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGAA GTGGACTAAAGCGTCGGATT 47.6 50.0 57.8 58.0 214-215 bp 1.0 73.9