Hotspot

psbK-psbI

LSC · 7866-8270 · 405 bp

Back to Petrocosmea

Pi 0.024

MarkerSeek score 66.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 405
variable_sites: 32
pi: 0.024
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GGGGGATTTTGTGATTGGGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	1008-1042 bp	1.0	85.4
psbK-psbI_p2	GGGGGATTTTGTGATTGGGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.0	58.0	1009-1043 bp	1.0	85.4
psbK-psbI_p3	TGGGGGATTTTGTGATTGGG	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	1009-1043 bp	1.0	85.4
psbK-psbI_p4	GGGGGATTTTGTGATTGGGT	CACTTTTCAGCCCTTCCCTT	50.0	50.0	58.0	58.0	1176-1212 bp	1.0	85.3
psbK-psbI_p5	TGGGGGATTTTGTGATTGGG	CACTTTTCAGCCCTTCCCTT	50.0	50.0	58.1	58.0	1177-1213 bp	1.0	85.3