Hotspot

ycf1

SSC · 123282-128639 · 5358 bp

Back to Petrocodon

Pi 0.0142

MarkerSeek score 49.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5358
variable_sites: 183
pi: 0.0142
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GATGTGGCGAACTAGCTTCA	CAATCCACCAAACTCCCACA	50.0	50.0	58.0	58.0	758-794 bp	1.0	86.4
ycf1_p2	TGATGTGGCGAACTAGCTTC	CAATCCACCAAACTCCCACA	50.0	50.0	58.0	58.0	759-795 bp	1.0	86.4
ycf1_p3	GATGTGGCGAACTAGCTTCA	TCCACCAAACTCCCACAAAG	50.0	50.0	58.0	57.9	755-791 bp	1.0	85.9
ycf1_p4	TGATGTGGCGAACTAGCTTC	TCCACCAAACTCCCACAAAG	50.0	50.0	58.0	57.9	756-792 bp	1.0	85.9
ycf1_p5	CAGGCAAATGAATGGGGTCT	CAATCCACCAAACTCCCACA	50.0	50.0	58.1	58.0	617-653 bp	1.0	85.8