Hotspot

petD

LSC · 76991-78230 · 1240 bp

Back to Petrocodon

Pi 0.0105

MarkerSeek score 54.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1240
variable_sites: 27
pi: 0.0105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCTCCGAAGAGAAAATGGAT	ACATGCCTATTATTTTACAATTGA	40.0	25.0	53.7	53.0	1291-1297 bp	0.8	33.8
petD_p2	TCTCCGAAGAGAAAATGGATT	ACATGCCTATTATTTTACAATTGA	38.1	25.0	54.6	53.0	1291-1297 bp	0.8	33.8
petD_p3	TCTCCGAAGAGAAAATGGAT	CATGCCTATTATTTTACAATTGAATCA	40.0	25.9	53.7	55.4	1290-1296 bp	0.8	33.8
petD_p4	TCTCCGAAGAGAAAATGGAT	ACATGCCTATTATTTTACAATTGAATC	40.0	25.9	53.7	55.4	1291-1297 bp	0.8	33.8
petD_p5	TCTCCGAAGAGAAAATGGATT	CATGCCTATTATTTTACAATTGAATCA	38.1	25.9	54.6	55.4	1290-1296 bp	0.8	33.8