Hotspot

trnA-UGC-rrn23

IRb · 104175-104326 · 152 bp

Back to Petrocodon

Pi 0.0289
MarkerSeek score 64.60
Primer available yes
Label trnA-UGC-rrn23

Feature Metadata

label_name
trnA-UGC-rrn23
feature_type
igs
region
IRb
length_bp
152
variable_sites
5
pi
0.0289
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-UGC-rrn23_p1 GTTCGAGTCCGCTTATCTCC GTCTCTGGGTGCCTAGGTAT 55.0 55.0 57.9 57.9 215-219 bp 1.0 81.6
trnA-UGC-rrn23_p2 GGTTCGAGTCCGCTTATCTC TCGATTACTACGCCCTTCCT 55.0 50.0 57.9 57.9 237-241 bp 1.0 81.5
trnA-UGC-rrn23_p3 GTTCGAGTCCGCTTATCTCC TCGATTACTACGCCCTTCCT 55.0 50.0 57.9 57.9 236-240 bp 1.0 81.5
trnA-UGC-rrn23_p4 GGTTCGAGTCCGCTTATCTC ATTCGGGAATCTCCGGATCT 55.0 50.0 57.9 58.0 288-292 bp 1.0 81.4
trnA-UGC-rrn23_p5 GTTCGAGTCCGCTTATCTCC ATTCGGGAATCTCCGGATCT 55.0 50.0 57.9 58.0 287-291 bp 1.0 81.4