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clpP-psbB

LSC · 72541-72995 · 455 bp

Back to Petrocodon

Pi 0.00923

MarkerSeek score 62.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 10
pi: 0.00923
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	551-558 bp	1.0	79.5
clpP-psbB_p2	CGCACTATACGTCAACCCAA	GGCCGGGATCATTCAATACA	50.0	50.0	58.0	57.7	583-590 bp	1.0	79.2
clpP-psbB_p3	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	554-561 bp	1.0	77.7
clpP-psbB_p4	TCCTCTCCAGGAATTCGGAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	522-529 bp	1.0	77.6
clpP-psbB_p5	CGTCAACCCAAGATGCATCT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.3	58.2	542-549 bp	1.0	77.5