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psbE-petL

LSC · 65541-66449 · 909 bp

Back to Petrocodon

Pi 0.0165

MarkerSeek score 58.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 909
variable_sites: 35
pi: 0.0165
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CATCGTAAGCCAAACCGGTA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1598-1617 bp	1.0	83.3
psbE-petL_p2	TACATCGTAAGCCAAACCGG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1600-1619 bp	1.0	83.3
psbE-petL_p3	ATCGTAAGCCAAACCGGTAC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1597-1616 bp	1.0	83.3
psbE-petL_p4	AAAGGATCAAAACGGCCTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1680-1699 bp	1.0	83.2
psbE-petL_p5	AAGGATCAAAACGGCCTGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1679-1698 bp	1.0	83.2