Hotspot

trnG-UCC-trnR-UCU

LSC · 10164-10372 · 209 bp

Back to Petrocodon

Pi 0.0817

MarkerSeek score 78.40

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 34
pi: 0.0817
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGGGACCGGTTTCTACTTCA	50.0	50.0	58.0	58.0	432-440 bp	1.0	89.7
trnG-UCC-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	GGAACATAGGGACCGGTTTC	50.0	55.0	58.0	58.0	439-447 bp	1.0	89.4
trnG-UCC-trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	AGGGACCGGTTTCTACTTCA	55.0	50.0	58.1	58.0	435-443 bp	1.0	89.4
trnG-UCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	TTTCTAGAGTGGGGTCTGCA	50.0	50.0	58.0	58.0	836 bp	0.2	47.6
trnG-UCC-trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	TTTCTAGAGTGGGGTCTGCA	55.0	50.0	58.1	58.0	839 bp	0.2	47.3