Hotspot

ycf1

SSC · 121381-126459 · 5079 bp

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Pi 0.386

MarkerSeek score 75.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5079
variable_sites: 1692
pi: 0.386
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGTAGAGCTATTCTGCGCA	AACAACCAGGAAGACGCAAT	45.0	45.0	57.0	58.0	945 bp	0.5	52.2
ycf1_p2	TTGTAGAGCTATTCTGCGCA	GACCGTCTCAAGGAAATCCC	45.0	55.0	57.0	58.0	1112 bp	0.5	52.1
ycf1_p3	TTGTAGAGCTATTCTGCGCA	GGACCGTCTCAAGGAAATCC	45.0	55.0	57.0	58.0	1113 bp	0.5	52.1
ycf1_p4	TTGTAGAGCTATTCTGCGCA	GGGACCGTCTCAAGGAAATC	45.0	55.0	57.0	58.0	1114 bp	0.5	52.1
ycf1_p5	TTGTAGAGCTATTCTGCGCA	AGGCATAATTCAAGGGCTCG	45.0	50.0	57.0	58.0	1342 bp	0.5	52.1