Hotspot

ndhI

SSC · 116874-117374 · 501 bp

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Pi 0.499

MarkerSeek score 80.90

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 231
pi: 0.499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGACCACGGAAGTTACTCCA	AGCCGAGTGTGAACGATTAC	50.0	50.0	58.0	58.0	1667 bp	0.5	59.9
ndhI_p2	AGACTTGTACAGACCACGGA	AGCCGAGTGTGAACGATTAC	50.0	50.0	58.0	58.0	1677 bp	0.5	59.9
ndhI_p3	AGACCACGGAAGTTACTCCA	GGTTCTGCAATAGGGATGGG	50.0	55.0	58.0	58.0	1829 bp	0.5	59.9
ndhI_p4	AGACTTGTACAGACCACGGA	GGTTCTGCAATAGGGATGGG	50.0	55.0	58.0	58.0	1839 bp	0.5	59.8
ndhI_p5	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1362 bp	0.5	59.5