Hotspot

ndhH

SSC · 119620-120804 · 1185 bp

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Pi 0.492

MarkerSeek score 80.00

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1185
variable_sites: 480
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CGTTGTTGTATACCCGCAGA	GGCAGTTGGGTATTCGAGAG	50.0	55.0	58.0	58.1	1494 bp	0.5	59.5
ndhH_p2	CGTTGTTGTATACCCGCAGA	GGGAGCCGTTTGAGTATGTT	50.0	50.0	58.0	57.9	1986 bp	0.5	59.1
ndhH_p3	CCTGACATAAGAAGGCCGAC	GGCAGTTGGGTATTCGAGAG	55.0	55.0	58.1	58.1	1758 bp	0.5	59.1
ndhH_p4	GACATAAGAAGGCCGACAGG	GGCAGTTGGGTATTCGAGAG	55.0	55.0	58.1	58.1	1755 bp	0.5	59.1
ndhH_p5	CGTTGTTGTATACCCGCAGA	TAGGGCAGTTGGGTATTCGA	50.0	50.0	58.0	58.1	1497 bp	0.5	58.9