Hotspot

ndhF

SSC · 107672-109897 · 2226 bp

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Pi 0.46

MarkerSeek score 80.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 891
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	TGTCGCTTGGTCATATCATTTG	55.0	40.9	57.9	57.1	2758 bp	0.5	35.8
ndhF_p2	TCGTTGTGGTCGGACTCTAT	TGTCGCTTGGTCATATCATTTG	50.0	40.9	58.2	57.1	2946 bp	0.5	35.6
ndhF_p3	ATCAATTCGGTCGTTGTGGT	TGTCGCTTGGTCATATCATTTG	45.0	40.9	57.8	57.1	2956 bp	0.5	35.5
ndhF_p4	TCTCTTCCTTCTCCGAGCTC	TGTCGCTTGGTCATATCATTTG	55.0	40.9	58.2	57.1	2888 bp	0.5	35.0
ndhF_p5	CTAGCACTGGGTAGACCTCA	GCCCAAATTTTTATCCTTGCTCT	55.0	39.1	57.9	57.9	2876 bp	0.5	34.1