Hotspot

ndhA

SSC · 117453-119618 · 2166 bp

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Pi 0.467

MarkerSeek score 79.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2166
variable_sites: 861
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCAATATACCTTGCGGC	AATCCGCCGCTTTGATTTTG	50.0	45.0	58.0	58.0	2632 bp	0.5	59.7
ndhA_p2	TGACCAATATACCTTGCGGC	TCAACAGGCCCTAGAAGGAA	50.0	50.0	58.0	58.0	2680 bp	0.5	59.4
ndhA_p3	TGACCAATATACCTTGCGGC	TTCAACAGGCCCTAGAAGGA	50.0	50.0	58.0	58.0	2681 bp	0.5	59.4
ndhA_p4	TGACCAATATACCTTGCGGC	GGAGTAGGCATTCTTGGTGG	50.0	55.0	58.0	58.0	2892 bp	0.5	59.3
ndhA_p5	AAACGCTCGGATGTGATCAA	AATCCGCCGCTTTGATTTTG	45.0	45.0	57.9	58.0	2719 bp	0.5	59.2