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rps15-ycf1

SSC · 121175-121380 · 206 bp

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Pi 0.459

MarkerSeek score 77.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 206
variable_sites: 90
pi: 0.459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGCAGACCTCTCTGAGATA	AATTATGGAACCTGCCCGTC	55.0	50.0	58.0	57.9	897 bp	0.5	59.4
rps15-ycf1_p2	AACACCATGCATTGATGGGT	AATTATGGAACCTGCCCGTC	45.0	50.0	58.1	57.9	1171 bp	0.5	59.1
rps15-ycf1_p3	CTCTCGAATACCCAACTGCC	AATTATGGAACCTGCCCGTC	55.0	50.0	58.1	57.9	993 bp	0.5	59.1
rps15-ycf1_p4	CCGCAGACCTCTCTGAGATA	TATGGAACCTGCCCGTCTAT	55.0	50.0	58.0	57.9	894 bp	0.5	59.0
rps15-ycf1_p5	CCTAATTGCTCTGGCCCATT	AATTATGGAACCTGCCCGTC	50.0	50.0	57.9	57.9	1365 bp	0.5	59.0