Hotspot

trnL-ccsA

SSC · 112008-112123 · 116 bp

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Pi 0.435

MarkerSeek score 76.80

Primer available yes

Label trnL-ccsA

Feature Metadata

label_name: trnL-ccsA
feature_type: igs
region: SSC
length_bp: 116
variable_sites: 47
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-ccsA_p1	GTGAAATCGGTAGACACGCT	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	531 bp	0.5	59.2
trnL-ccsA_p2	GTGAAATCGGTAGACACGCT	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.0	58.1	532 bp	0.5	59.2
trnL-ccsA_p3	TGAAATCGGTAGACACGCTG	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	530 bp	0.5	59.2
trnL-ccsA_p4	TGAAATCGGTAGACACGCTG	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.0	58.1	531 bp	0.5	59.2
trnL-ccsA_p5	GTGAAATCGGTAGACACGCT	TGATCCACAAAGAAGAGCCG	50.0	50.0	58.0	57.9	649 bp	0.5	59.1