Hotspot

rpl32-trnL

SSC · 111124-111927 · 804 bp

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Pi 0.434

MarkerSeek score 79.80

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 804
variable_sites: 284
pi: 0.434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGCAGCGTTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	921 bp	0.5	58.9
rpl32-trnL_p2	GGCAGCGTTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	922 bp	0.5	58.9
rpl32-trnL_p3	AAAGGATATTGGGCAGCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	932 bp	0.5	58.8
rpl32-trnL_p4	AAAGGATATTGGGCAGCGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	933 bp	0.5	58.8
rpl32-trnL_p5	GGCAGCGTTAAAAGCTTTGT	GAAGTAGCAAAGCCTTGGGT	45.0	50.0	57.9	58.1	1432 bp	0.5	58.2