Hotspot

ndhF-rpl32

SSC · 109898-110958 · 1061 bp

Back to Petasites

Pi 0.453

MarkerSeek score 81.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1061
variable_sites: 407
pi: 0.453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ATATCTGCCCTTCCCGAGAA	50.0	50.0	58.0	57.9	1584 bp	0.5	58.9
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	ATATCTGCCCTTCCCGAGAA	45.0	50.0	58.0	57.9	1582 bp	0.5	58.8
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	ATATCTGCCCTTCCCGAGAA	45.0	50.0	58.0	57.9	1583 bp	0.5	58.8
ndhF-rpl32_p4	TACTTTTTGTTGCCGTCGGA	ATATCTGCCCTTCCCGAGAA	45.0	50.0	58.0	57.9	1592 bp	0.5	58.8
ndhF-rpl32_p5	GTTGCCGTCGGAAAAAGAAG	TGCCCTTCCCGAGAAAGATA	50.0	50.0	58.0	58.1	1579 bp	0.5	58.7