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rps8-rpl14

LSC · 79123-79346 · 224 bp

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Pi 0.199

MarkerSeek score 84.00

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 29
pi: 0.199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ACTCGAACCACGCTTTTTCT	GAACTCGAGTTTTTGGTGCG	45.0	50.0	58.1	58.0	350-387 bp	1.0	89.7
rps8-rpl14_p2	ACTCGAACCACGCTTTTTCT	CGATTGCCCGAGAATTGAGA	45.0	50.0	58.1	58.1	332-369 bp	1.0	89.7
rps8-rpl14_p3	ACTCGAACCACGCTTTTTCT	TTGCCCGAGAATTGAGACAG	45.0	50.0	58.1	57.9	329-366 bp	1.0	89.5
rps8-rpl14_p4	ACTCGAACCACGCTTTTTCT	GCCCGAGAATTGAGACAGTT	45.0	50.0	58.1	57.9	327-364 bp	1.0	89.5
rps8-rpl14_p5	ACTCGAACCACGCTTTTTCT	ACTCGAGTTTTTGGTGCGAT	45.0	45.0	58.1	58.1	348-385 bp	1.0	89.0