Hotspot

rpoA

LSC · 78734-79741 · 1008 bp

Pi 0.00694

MarkerSeek score 62.70

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 7
pi: 0.00694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGAATCTATGCCGATTCCGA	CATAATGGCTGTAGACCCCC	45.0	55.0	56.5	57.4	1228-1230 bp	1.0	64.0
rpoA_p2	TGAATCTATGCCGATTCCGAA	CATAATGGCTGTAGACCCCC	42.9	55.0	57.3	57.4	1228-1230 bp	1.0	62.0
rpoA_p3	TGAATCTATGCCGATTCCGA	ATAATGGCTGTAGACCCCCG	45.0	55.0	56.5	58.9	1227-1229 bp	1.0	61.0
rpoA_p4	ATGAATCTATGCCGATTCCGA	CATAATGGCTGTAGACCCCC	42.9	55.0	57.0	57.4	1229-1231 bp	1.0	60.2
rpoA_p5	TGAATCTATGCCGATTCCGAA	ATAATGGCTGTAGACCCCCG	42.9	55.0	57.3	58.9	1227-1229 bp	1.0	59.0