Hotspot

ccsA

SSC · 114710-115678 · 969 bp

Back to Pertya

Pi 0.00413
MarkerSeek score 47.00
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
969
variable_sites
4
pi
0.00413
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GTGAAATCGGTAGACACGCT GTTTTTGATTCTGGACCGCG 50.0 50.0 58.0 58.0 1513-1542 bp 1.0 80.4
ccsA_p2 GTGAAATCGGTAGACACGCT AGAACCGCGTGAATCACTAC 50.0 50.0 58.0 58.0 1261 bp 1.0 80.3
ccsA_p3 TGAAATCGGTAGACACGCTG AGAACCGCGTGAATCACTAC 50.0 50.0 58.0 58.0 1260 bp 1.0 80.2
ccsA_p4 GTGAAATCGGTAGACACGCT TCTCGCCGGTTGACATAAAG 50.0 50.0 58.0 58.0 1222 bp 0.5 59.9
ccsA_p5 TGAAATCGGTAGACACGCTG TCTCGCCGGTTGACATAAAG 50.0 50.0 58.0 58.0 1221 bp 0.5 59.9