Hotspot

rps15-ycf1

SSC · 123705-125304 · 1600 bp

Back to Pertya

Pi 0.00895
MarkerSeek score 65.80
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
1600
variable_sites
14
pi
0.00895
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGTCTCCGTATCTTATTGGTGA ACAGATAGAAAATATTTCGGTTGGA 40.9 32.0 56.1 56.6 1771-1833 bp 1.0 43.4
rps15-ycf1_p2 TGGTGAAACTGACTACTTGA ACAGATAGAAAATATTTCGGTTGGA 40.0 32.0 53.9 56.6 1755-1817 bp 1.0 43.4
rps15-ycf1_p3 AAGTCTCCGTATCTTATTGGTGA ACAGATAGAAAATATTTCGGTTGGA 39.1 32.0 56.8 56.6 1772-1834 bp 1.0 43.4
rps15-ycf1_p4 AGTCTCCGTATCTTATTGGTGAA ACAGATAGAAAATATTTCGGTTGGA 39.1 32.0 56.8 56.6 1771-1833 bp 1.0 43.4
rps15-ycf1_p5 AGTCTCCGTATCTTATTGGTGA ACAGATAGAAAATATTTCGGTTGG 40.9 33.3 56.1 55.3 1771-1833 bp 1.0 43.4