Hotspot

ndhG-ndhI

SSC · 119062-119432 · 371 bp

Back to Pertya

Pi 0.0238
MarkerSeek score 47.80
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
371
variable_sites
5
pi
0.0238
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.57
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AGACCACGGAAGTTACTCCA GATGGACTTTACCGAGGCTG 50.0 55.0 58.0 58.1 1219-1379 bp 1.0 84.0
ndhG-ndhI_p2 AGACTTGTACAGACCACGGA GATGGACTTTACCGAGGCTG 50.0 55.0 58.0 58.1 1229-1389 bp 1.0 83.9
ndhG-ndhI_p3 AGACCACGGAAGTTACTCCA TTGATCACATCCGAGCGTTT 50.0 45.0 58.0 57.9 884-1044 bp 1.0 83.9
ndhG-ndhI_p4 AGACTTGTACAGACCACGGA TTGATCACATCCGAGCGTTT 50.0 45.0 58.0 57.9 894-1054 bp 1.0 83.8
ndhG-ndhI_p5 AGACCACGGAAGTTACTCCA AGATGGACTTTACCGAGGCT 50.0 50.0 58.0 58.1 1220-1380 bp 1.0 83.4