Hotspot

rpl32-trnL-UAG

SSC · 113620-114522 · 903 bp

Back to Pertya

Pi 0.0182
MarkerSeek score 69.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
903
variable_sites
16
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGAAAGGATATTGGGCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 1016-1036 bp 1.0 80.0
rpl32-trnL-UAG_p2 AAGGAAAGGATATTGGGCGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 1017-1037 bp 1.0 80.0
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 999-1019 bp 1.0 79.8
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 1000-1020 bp 1.0 79.8
rpl32-trnL-UAG_p5 AAGGAAAGGATATTGGGCGG TTAGAAGATGACATGCCGCC 50.0 50.0 57.6 58.0 1080-1100 bp 1.0 79.5