Hotspot

ycf4-cemA

LSC · 61701-62001 · 301 bp

Back to Pertya

Pi 0.0337
MarkerSeek score 58.40
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
301
variable_sites
10
pi
0.0337
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 GAGCCATTCCATTGACTCGT GAGAAATCCACCAGGGCAAA 50.0 50.0 58.0 58.1 481-1040 bp 1.0 80.7
ycf4-cemA_p2 GAGCCATTCCATTGACTCGT GGAATTCCTGAGCCCAAGAG 50.0 55.0 58.0 58.0 832-1391 bp 1.0 80.4
ycf4-cemA_p3 GGGATCGCGGGTCTTTTTAT GAGAAATCCACCAGGGCAAA 50.0 50.0 58.0 58.1 722-1281 bp 1.0 80.3
ycf4-cemA_p4 GGGATCGCGGGTCTTTTTAT GGAATTCCTGAGCCCAAGAG 50.0 55.0 58.0 58.0 1073-1632 bp 1.0 80.2
ycf4-cemA_p5 CGTTGGGGATTTCCTGGAAA GGAATTCCTGAGCCCAAGAG 50.0 55.0 58.1 58.0 965-1524 bp 1.0 79.9