Hotspot

accD-psaI

LSC · 60098-60633 · 536 bp

Back to Pertya

Pi 0.0225
MarkerSeek score 58.10
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
536
variable_sites
12
pi
0.0225
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCCACACTTTCTTTCCTTTGA AAAGAAGCCATTGCGATTGC 38.1 45.0 55.3 58.0 645-815 bp 1.0 51.6
accD-psaI_p2 TCCACACTTTCTTTCCTTTGAA AAAGAAGCCATTGCGATTGC 36.4 45.0 56.0 58.0 645-815 bp 1.0 49.9
accD-psaI_p3 TCCACACTTTCTTTCCTTTGA TTGCGATTGCCGGAAATACT 38.1 45.0 55.3 58.3 635-805 bp 1.0 49.6
accD-psaI_p4 CCACACTTTCTTTCCTTTGAATCA AAAGAAGCCATTGCGATTGC 37.5 45.0 57.9 58.0 644-814 bp 1.0 48.4
accD-psaI_p5 TCCACACTTTCTTTCCTTTGA GCCGGAAATACTAGGCCTAC 38.1 55.0 55.3 57.5 627-797 bp 1.0 48.0