Hotspot

trnF-GAA-ndhJ

LSC · 49311-49985 · 675 bp

Back to Pertya

Pi 0.0136
MarkerSeek score 68.90
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
675
variable_sites
8
pi
0.0136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACG 50.0 50.0 58.1 56.8 695-778 bp 1.0 74.7
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA GGATAGGATGGCCTTTACGT 50.0 50.0 58.1 56.8 694-777 bp 1.0 74.7
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGATGGCC 50.0 47.6 58.1 57.7 702-785 bp 1.0 74.1
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA TTGGATAGGATGGCCTTTACG 50.0 47.6 58.1 57.5 696-779 bp 1.0 72.6
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACGT 50.0 47.6 58.1 58.6 695-778 bp 1.0 71.7