Hotspot

trnE-UUC-rpoB

LSC · 13350-14234 · 885 bp

Back to Pertya

Pi 0.0113
MarkerSeek score 62.70
Primer available yes
Label trnE-UUC-rpoB

Feature Metadata

label_name
trnE-UUC-rpoB
feature_type
igs
region
LSC
length_bp
885
variable_sites
10
pi
0.0113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rpoB_p1 GAAGTCGTAACCACTGGACC AGTTCTTCTGTCAAACCCTGA 55.0 42.9 57.9 56.8 993-1030 bp 1.0 62.6
trnE-UUC-rpoB_p2 TAACCACTGGACCATAGGGG AGTTCTTCTGTCAAACCCTGA 55.0 42.9 58.1 56.8 986-1023 bp 1.0 62.3
trnE-UUC-rpoB_p3 AACCCTCGTTATCTCCGTGA AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.2 56.8 1019-1056 bp 1.0 62.0
trnE-UUC-rpoB_p4 ACCCTCGTTATCTCCGTGAA AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.2 56.8 1018-1055 bp 1.0 62.0
trnE-UUC-rpoB_p5 TGAACCCTCGTTATCTCCGT AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.2 56.8 1021-1058 bp 1.0 62.0