Hotspot

petN-psbM

LSC · 11464-12012 · 549 bp

Back to Pertya

Pi 0.00399
MarkerSeek score 63.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
549
variable_sites
2
pi
0.00399
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTT 50.0 40.0 58.2 55.6 663-711 bp 1.0 63.6
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTTT 50.0 38.1 58.2 56.4 663-711 bp 1.0 61.9
petN-psbM_p3 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATTT 50.0 40.0 57.1 55.6 664-712 bp 1.0 57.7
petN-psbM_p4 CTCGCTTGGGCTGCTTTAAT TGCTACTGCACTGTTCATTT 50.0 40.0 58.9 55.6 655-703 bp 1.0 57.6
petN-psbM_p5 TGGGCTGCTTTAATGGTAGTC TGCTACTGCACTGTTCATTT 47.6 40.0 58.0 55.6 649-697 bp 1.0 56.9