Hotspot

psaJ-rpl33

LSC · 67399-67887 · 489 bp

Back to Persicaria

Pi 0.0719

MarkerSeek score 78.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 489
variable_sites: 135
pi: 0.0719
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACCCGTGACATTTTGTACCC	GCCTACGAAAAGACCGCTTA	50.0	50.0	58.1	58.0	1565-1757 bp	0.923077	88.1
psaJ-rpl33_p2	CCCGTGACATTTTGTACCCA	GCCTACGAAAAGACCGCTTA	50.0	50.0	58.1	58.0	1564-1756 bp	0.923077	88.1
psaJ-rpl33_p3	TTTTCCCGGATGCGTTAACA	GCCTACGAAAAGACCGCTTA	45.0	50.0	58.1	58.0	1001-1214 bp	0.923077	88.1
psaJ-rpl33_p4	ACAGGATTTGAACCCGTGAC	GCCTACGAAAAGACCGCTTA	50.0	50.0	58.1	58.0	1576-1768 bp	0.923077	88.1
psaJ-rpl33_p5	GGATGCGTTAACATTCCCCT	GCCTACGAAAAGACCGCTTA	50.0	50.0	57.9	58.0	1065-1207 bp	0.769231	82.3