Hotspot

ycf3-trnS-GGA

LSC · 44404-45266 · 863 bp

Back to Persicaria

Pi 0.0538

MarkerSeek score 80.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 213
pi: 0.0538
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	GCGTGGGAGCTTTTGTTAAC	ATTGGGTATGGCTCCGACTA	50.0	50.0	58.0	57.9	2064-2268 bp	1.0	90.9
ycf3-trnS-GGA_p2	GCGTGGGAGCTTTTGTTAAC	TGGGTATGGCTCCGACTATT	50.0	50.0	58.0	57.9	2062-2266 bp	1.0	90.9
ycf3-trnS-GGA_p3	GCGTGGGAGCTTTTGTTAAC	TTGGGTATGGCTCCGACTAT	50.0	50.0	58.0	57.9	2063-2267 bp	1.0	90.9
ycf3-trnS-GGA_p4	GCGTGGGAGCTTTTGTTAAC	ACCCACCGACATTGTTATGG	50.0	50.0	58.0	57.9	1962-2166 bp	0.923077	87.6
ycf3-trnS-GGA_p5	CCAAGGTACGAACGAGATGG	TGGGTATGGCTCCGACTATT	55.0	50.0	58.1	57.9	1927-2131 bp	0.769231	81.4